A method to construct 12 kb plasmid vector 重组质粒载体的方法
Second round pcr products were cloned to t / a plasmid vectors to set up the subtractive library Westernblot分析了nadp一苹果酸酶蛋白合成随盐胁迫的变化情况。
Rynai - w and xynb1 were all cloned into the plasmid vector ppic9 q , and were all overexpressed in pichia pastoris gs l 1 5 将xynb1序列克隆进酵母表达载体ppic9上,在毕赤酵母gs115中得到高效特异性表达。
Result , we succeed in doing t - pa gene from fetal lung and construct a sort of eukaryon expression plasmid vector with pcdna3 . 1 ( + ) 结果:成功地从胎儿肺组织中克隆了t - pa基因,并构建了以pcdna3 . 1 ( + )为载体的真核表达质粒载体。
Put this fusion gene under the control of the promoter of ie1 gene , and then an eucaryotic cell expression plasmid vector pgem / ie1 " - gfp - actin resulted 将该融合基因置于ie1基因启动子的控制之下,构建成了真核表达质粒pgem ie1 - gfp - actin 。